Immunoassay enzyme ukuhlolwa kwegazi

ukuhlaziywa Immunoassay igazi yindlela laboratory esetshenziswa kwezokugoma ekunqumeni okuqukethwe ambalwa kanye qualitative we ezahlukene amagciwane, compounds, nezinye macromolecules. Lolu cwaningo lusekelwe ekhethekile ukuphendula eqondene amasosha omzimba antigen. Enzyme test immunoassay igazi kwanele kabanzi imithi yesimanje. eziyinkimbinkimbi Kumiswa ene usebenzisa njengoba angukhiye ethile ukusabela kwamakhemikhali kanye enzyme.

ukuhlolwa Immunoassay igazi wembula lokuhlushwa omzimba. Nale cwaningo kuba nokwenzeka ukucacisa izakhiwo immunological, immunoglobulin, amazinga ama-hormone, lokuhlushwa nezinye izingxenye eziphila abakhuthele. Ukuze zocwaningo laboratory usebenzisa amniotic fluid, igazi, amahlaya Vitreous, futhi cerebrospinal fluid.

Kwezinye izimo, le -enzyme-exhumene Assay immunosorbent (ELISA) libhekwa ngokuvumelana uhlobo ukuxhumana immunochemical. Lokhu kubhekwa esigabeni sokuqala kulolu cwaningo, lapho element esibophezelayo azimisele. Endabeni ka kuphela omzimba athile futhi antigen, indlela ubhekwa non-ukuncintisana. Uma Immunoassay ukuhlaziywa igazi esigabeni sokuqala sabonisa okuqukethwe ngesikhathi esisodwa enkompolo ukuhlolwa kanye Analogue yayo, ekhuthaza akasakwazi ngo lwesigaba okuqinile noma iprotheni alebuliwe, kubhekwa eqolo.

ELISA indlela iye kabanzi hhayi kuphela emkhakheni wamuva wezokwelapha, kodwa futhi ezolimo, umkhakha yemvelo, kanye ucwaningo lwesayensi. Kufanele kuqashelwe ukuthi, kanye nezinye izindlela zocwaningo immunochemical, lokhu ukuhlaziywa inika Imiphumela zamanga engemihle futhi okungamanga. It kuxhomeke ezicini eziningi. Ngokwesibonelo, lapho anti- immunoglobulin G uqondisa omzimba immunoglobulin M, sinalo isici samathambo.

Kufanele kuqashelwe ukuthi namuhla ELISA indlela kunezinzuzo eziningi. Isikhathi esiningi, basuke eboniswe umnqopho ngenxa ezimali okuzenzakalelayo imiphumela, isivinini kanye lula. Enye yezinto ezibaluleke kakhulu kwi-ekuhlonzweni nasekwelashweni Izibikezelo izifo yikhono ukutadisha immunoglobulin, ayisiza amakilasi ezahlukene. Ngo inqubo yokwelapha yesimanje, enzyme immunoassay kubhekwa eziyisisekelo amasu laboratory pathologies.

ELISA igazi iwela isigaba ukuhlolwa serological. Kubalulekile ikakhulukazi endabeni ukwehluleka ukuhlonza ejenti causative.

Ekukhombeni omzimba athile esungulwe ukwandisa titers yabo. Ukutadisha sera abhanqiwe abazenzi ngezinhloso zokuxilonga nge esingaba amasonto amabili kuya kwamathathu. Ukunqunywa immunoglobulin amakilasi esasivumele ukuba sinikeze incazelo ecacile zonke izigaba inqubo ukutheleleka. isilawuli Dynamic of titer yabo futhi ungafaka isicelo ukuphathwa sicabangela izigaba kusungulwa.

Ukuhlonza antigen nakho kubalulekile. Ukuzimisela kwabo senziwa ekuqaleni kwalesi sifo. Lokhu kuvumela uphethwe okusheshayo. Enzyme immunoassay ukuhlolwa igazi, ukuqaqwa Kwenziwa ngokuya ambalwa, ukuze nokuhlola ukusebenza ukuphathwa isicelo.

ELISA Igazi ingenye ucwaningo enkulu ukuthola of STD (izifo ezithathelwana ngocansi). Kuyadingeka ukuba agcizelele ukubaluleka kwayo ekuhlonzeni HIV kanye nogcunsula.

Iqhaza ejenti izifo zangasese, ingqondo izilonda ezingxenyeni ezahlukene insikazi uhlelo zokuzala, uye wanda kakhulu kule minyaka yamuva. Ngakho-ke kubhekwa igciwane okuthiwa ukuxubana eziyingozi kunazo zonke namuhla. Kulokhu, lo amagciwane ngaphezulu. Lokhu ikakhulukazi nzima ukuhlonza. Kulokhu, ukuze afeze uphenyo isetshenziswa ELISA indlela.