Kanjani i-cryopreservation ye-sperm

Ukwehlukaniswa okufakelwayo , ukuguqulwa kwe-sperm kuyadumeka. Ngosizo lwesitoreji sesikhathi eside sokushisa komzimba, iVIV ilula. Umsebenzi kanye nokusebenza kwe-ejaculate kugcinwa ngezindleko zokushisa okuphansi - ukupholisa kuyenzeka nge-nitrogen enamandla. Indlela yokugcina entsha ivumela ukutholakala kwezinsizakalo ezincane ngisho nangemva kweminyaka eyishumi.

Kungani udinga umphumela wokuqandisa

Ukwelashwa kwe-spropreservation yesidakamizwa kunconywa kubantu njengesixazululo sokungabikho kokuzala. Indlela iba indawo yabesifazane abangafuni ukuxhumana nomuntu ogulayo. Ukuqhwala kubalulekile uma kunengozi yokudluliselwa kwesifo, kodwa akukho usongo lomntwana ozayo.

Amadoda angaziqinisekisa esikhathini esizayo ngezinsongo zempilo ezikhona:

• Izinkinga zezifo ezingapheli zesistimu ye-genitourinary;
• I-oncology ethuthukile;
• Ukuhlinzwa okuzayo ngemva kokulimala;
• Ukukhubazeka.

Abantu bafuna ukulondoloza izinto eziphilayo ngaphambi kwemisebenzi ezayo, imisebe noma i-chemotherapy. Futhi, amadoda akhetha izindlela zokuqandisa i-laboratory ukuze aqiniseke owesifazane, ngaphambi kokuya emsebenzini eseengozini enkulu yokuphila.

Indlela ye-vitrification igcina izinto eziphilayo:

• Isibungu;
• Imibungu;
• Amaseli egazi;
• Izitho zangaphakathi;
• Amaqanda.

Ukwehliswa kwesidoda eMoscow kuthola ukuthandwa ngenxa yokutholakala. Imitholampilo eminingi inikeza izinsizakalo zokulondoloza izinto eziphilayo. Abantu baqala ukusebenzisa inqubo njengomthombo wemali engenayo esimweni sempilo enhle yomzimba. Abaxhasi badinga abesifazane nabashadile abahlukunyezwa.

Izinqubo Zokusekela

Amaseli avikelwe ekubhujisweni yizinto ezikhethekile - i-cryoprotectant. Ngaphandle kokusetshenziswa kwezinto ezikhethekile, ukukhanda kwakungeke kuphumelele kakhulu.

Kodwa ngokuphila okuphilayo, lapho ukwenziwa kwe-spyopreservation ye-sperm kwenziwa, izinto ezimbi nazo zithonya:

• Ukwakhiwa kwamakhekhedi e-ice ngaphakathi kweseli kusuka kumanzi wamaseli;
• Njengomphumela wesici sokuqala, ukuphelelwa amandla kwamanzi kuyabonakala.

Ukuvimbela ukubhujiswa kwamangqamuzana aphilayo, zifakwa ezixazululweni zama-cryoprotectants. Izinto zokuvikela zihlukaniswe ezilandelayo:

• Ukungenelela - ukuqeda uketshezi ketshezi ngaphakathi kwamaseli;
• Ukungena-ukungenelela - kuba nje ukungezwa kohlobo lokuqala lokuvikelwa, inhloso yalo ukuvikela iseli ezintweni zangaphandle ezingalungile.

Iqembu lokuqala lihlanganisa: ethylene glycol, dimethylsulfoxide, propylene glycol. Izinto ezengeziwe ziyi-sucrose, i-ficoll. Inqubo eguquguqukayo yokungafaki isisindo ihambisana nezinyathelo zokukhulula amangqamuzana aphilayo ahlobene nezixhumanisi ze-cryoprotectants.

Ukulungiselela nokuziphatha kwenqubo

Ngaphambi kokuba u-spyopreservation of sperm iqhutshwe, ukuhlolwa okuphelele kwesimo seminikelo yabanikeli kwenziwa. Ukuhlaziywa okuyinhloko kokuhlaziywa kwamanzi omzimba okuba semthunjini kuba umuthi. Emtholampilo, kuhlolwa isilingo ukuze kufakwe indaba yomuntu. Lokhu kudingeke ukuqinisekisa ukuthi ikhono lembewu lizophola ngemuva kokugcina isikhathi eside.

I-cryotest okuthiwa iyasiza odokotela ukuhlola isiqinisekiso sokuthola umuthi onempilo. Ukuhlolwa okungeziwe kuhloswe ukuvikela ingane kanye nomama esikhathini esizayo ezifweni:

• I-Herpes simplex virus;
• I-Hepatitis B no-C;
• Ukutheleleka nge-HIV;
• I-Syphilis.

Ngesicelo sekhasimende, ucwaningo oluningi oluphelele lwabaxhasi lungenziwa. Uvivinyo luqhutshwa imizuzu engu-20 ngemuva kwenqubo yokulimaza. Uma zonke izinyathelo zokuhlolwa zazihle, i-semen ixubene nezixazululo zama-cryoprotectants futhi ifakwe kwisitoreji esikhethekile.

Indlela i-biomaterial egcinwa ngayo

Kulezi ziqukathi lapho kutholakala khona uketshezi oludlulayo lwe-seminal, lokushisa kuka -196 ° C kufanele kugcinwe. Izimo ezifanelekayo zitholakala ngezinto ezithile - i-nitrogen ene-liquid.

I-cryopreservation ye-sperm yomuntu eyenziwa ngamatayipi wokuhlolwa kwe-polypropylene (imikhumbi ye-Dewar), lapho i-biomaterial igcinwa khona. I-capsule ngokwayo ifakwa kwisitsha esine- nitrogen enamandla .

Isitoreji sesikhathi eside sithatha iminyaka emithathu, ngalesi sikhathi isivumelwano nesihlinzeki senziwa. Kuvunyelwe ukuhambisa izitsha kuminye imitholampilo ngokuqonda kwamakhasimende. Ukuze wenze lokhu, kuzodingeka uqinisekise ngokubhala isifiso sokuthutha isisindo seqhwa. Isiguli sinikezwa idokhumenti ngesikhathi sokukhululwa kwe-biomaterial.

Isikhathi esihle sokugcina i-ejaculate sisungulwe, esilingana neminyaka emithathu. Isikhathi esiphezulu asiqinisekisi ukulondolozwa kwe-quality biomaterial, ngoba inqubo yezinguquko zangaphakathi kumaseli iqala. Umuthi awusenalo ikhono lokushisa okungapheli, ngakho-ke umnikezeli unquma noma ukugcina insimu emgqonyeni, noma ukuphinda inqubo yokulondoloza.

Yini enquma izindleko zesevisi

Ukusabalalisa kweziphakamiso ezivela emitholampilo yangasese kuphakamisa inkinga yokukhetha inhlangano ethembekileyo eqhuba inqubo yekhwalithi. Imitholampilo ingasebenzisa indlela yendabuko yokuqandisa - lokhu kuhamba kancane kwe-spyopreservation yesidoda. Inani lentengo enjalo lihlukile kancane, kepha kunezingozi zokulimaza i-ejaculate. Ngokuvamile kunendlela yokwenza i-vitrification - inqubo yokulondolozwa kwe-ultrafast ye-biomaterial.

Izindleko ezilinganiselwe zesevisi echazwe yi-ruble ayi-10 400. Futhi ihlukahluka kuye ngokuthi izici ezilandelayo:

• Udumo lomtholampilo, inani lezinsizakalo ezinikeziwe kanye nempendulo emihle;
• Indlela ye-cryopreservation (i-slowly or vitrification) encane;
• Ithenda yesivumelwano - ngokukhokha okugcwele isikhathi eside, izindleko ziyancishiswa ngokuphawulekayo;
• Izimo zokugcina (esiteshini esivamile noma esitsheni esihlukile).

Ukuze ukugcinwa kwemibungu inyanga eyodwa kuzodingeka ukhokhe ama-ruble angu-1,000., Futhi ukumiswa kwezinto ezigcinwe kwisitolo sokugcina isikhathi esifanayo kuzobiza izindlu ezingu-4,000.

Umlando wendlela okwenzeka ngayo iqhwa

Ukuthembela kule nqubo kwazalwa ngemva kokusungulwa kweli cell kanye nokuzalwa kwengane enempilo eNetherlands. Lezi zenzakalo zivela ngo-1984, lapho ukuhlolwa kwe-spyopreservation kwe-sperm kwahlolwa ngempumelelo. Ubuchwepheshe buye basinda esikhathini sethu, kodwa kuye kwaba nokuthuthukiswa okuphawulekayo ekusebenzeni kokuqandisa.

Ukusatshalaliswa kokufakelwa kwamanje kuyabangela imizwelo emibi emphakathini. Imibuzo ephuthumayo kakhulu yilezi:

• Ukubhaliswa kwamalungelo angokomthetho wengane, ukusungulwa kokubeletha nokuzala;
• Uhlangothi lokuziphatha lokukhulelwa luhlala lungavamile;
• Umphakathi wamaqembu athile asempumalanga awukulungele indlela entsha yokuphila.

Kodwa, naphezu kwezinkinga ezikhona, abantu baqhubeka bezama ukuguqulwa njengendlela yokulondoloza impilo yengane ezayo.