Vitrification ka oocytes: kuyini?

Eminyakeni yamuva, e kokukhulelwa kwesibeletho okhula ngokushesha okukhulu. Le nqubo ayisekho kubhekwa ukuba into Amazing futhi zingakwazi ukufunda abantu abavamile. IVF Kwenziwa emhlabeni wonke. laboratories Akhethekile kanye nemitholampilo ayatholakala kuwo wonke amadolobha amakhulu. Minyaka yonke ukuthuthukisa entsha, izindlela ngcono okuzala. Through ukuthuthukiswa le ndlela, imibhangqwana eminingi nenzalo bakwazi babe abazali. Ngokuvamile ukuqoqwa kolwazi mayelana IVF, abesifazane babhekene eside ezifana vitrification ka oocytes. Le nkulumo ibhekisela inqubo empeleni esasisesisha elikuvumela ukuba ngcono ikhwalithi amaqanda.

Umqondo "oocyte vitrification" - kuyini?

Ukuze uqonde ukuthi inqubo vitrification, kumele ube umqondo wokuthi kwesibeletho fertilization. Le nqubo ihilela zalokho zesifazane germ cells (oocytes) kusukela amaqanda. Khona-ke kukhona yokuhlanza kanye kokukhulelwa yabo isidoda donor noma umlingani (uma umyeni asikwazi ukuba izingane). Emva kwalokho, i isabelo zygote ibekwa ingaphakathi lesizalo. Vitrification ka oocytes - inqubo efanayo kuze cryopreservation ka oocytes. Nokho, kuyinto okuthuthukile. Ngaphansi vitrification kwakusho okokuqandisa beya germ cells. Ngenxa yalokho, i-oocytes zingagcinwa isikhathi eside. Naphezu "eqandisiwe" isimo, amaseli Vitrified iphila. Le nqubo ubhekwa umgoqo omkhulu kwezokwelapha, ngoba sibonga abesifazane wakhe bayakwazi ukuba nomntwana, ngisho nalapho engekho Izitho zokuzala.

Yini injongo oocyte vitrification Kwenziwa?

EYurophu lehla ngokushesha inani imiqondo ezifana cryopreservation, vitrification zemibungu bese oocytes, bebelokhu baziwa isikhathi eside. Lokhu kungabonwa ifilimu entshonalanga phezu imithi. Emazweni ayengaphansi kweSoviet Union, futhi, odokotela ujwayelene migomo amashumi eminyaka. Nokho, lezi zindlela ziye zaba kuyatholakala ezigulini muva. Ezimweni eziningi, vitrification ka oocytes ekhiqizwa for ngaphandle kwesibeletho fertilization. Phakathi okokuqandisa amaseli beya ngendlela owesifazane enezinwele usethubeni elihle IVF yimpumelelo. Batch lokuqala oocytes ekhethiwe kusukela esibelethweni abakwazi sizakhele. Kulesi kabusha ezimbalwa impahla kungadingeki, njengoba kukhona amaseli kakade Vitrified. Ngaphezu kwalokho, kukhona amacala lapho pair oyinyumba icela kulondolozwe oocytes isikhathi eside. ngokuvamile kudingeka ezimweni ezihlukahlukene sokugembula owesifazane angaphansi kwazo lapho asikwazi ukuba izingane. Futhi, wenza lesi sicelo anjalo abhekisela iziguli ukulungiselela ukuhlinzwa ukhishwe Izitho zokuzala.

Ngaphansi akukho pathologies ukuncoma amaqanda vitrification?

Vitrification ka oocytes kanye imibungu - inqubo ikhokhwe singatholwa yinoma ubani. izinkomba okubalulekile ayinayo, nokho, kubalulekile kuphela ezindaweni ezithile. Lokhu akusho ukuthi abanye besifazane abakwazi ukukhokhela vitrified kangakanani ezitokisini zabo. Noma kunjalo, ngenxa yokuntuleka kwezinhlelo ezibanzi izizathu lo okokuqandisa oocytes ngendlela efanayo iziguli ukuncoma cryopreservation. Ngokuvamile vitrification uqhutshwa ngaphansi kwale mibandela elandelayo:

1. Ilungiselela ovariectomy. Lo msebenzi wenziwa umdlavuza wesibeletho kanye ngiboshelwa ukusolwa ngokushushumbisa ukuthuthukiswa yayo. Lokhu isizathu akuyona yande kakhulu, kusukela ezikhathini eziningi, izifo zabesifazane umdlavuza pathologies isuswe bese isibeletho.
2. Ukungakwazi ukuthola isidoda engayitholi nsuku zonke oocytes. Ngenxa yalesi sizathu, amaseli kufanele iqandiswe inqobo nje uma impahla ukuvundisa ngeke anikezwe.
3. Umdlavuza kwanoma iyiphi kwasendaweni. Kungakhathaliseki lapho has a umdlavuza idinga ngemisebe ukwelashwa chemical (cytostatic) izidakamizwa. Ngayinye yalezi zindlela engathandeki imiphumela sokukhulelwa. Ngemva kwalezi ukwelashwa ukubhubhisa umdlavuza hhayi kuphela, kodwa futhi amaseli okunempilo, kuhlanganise oocytes.
4. isifiso sowesifazane ukuba babe nezingane esikhathini esikude, isibonelo, ngemva kokuma ukuya esikhathini. Ezimweni ezinjalo azivamile cryopreservation ayitheli ngoba Iqanda umnkantsha ngale ndlela iphila isikhathi eside. Ngakho-ke, odokotela basikisela indlela ehlukile - vitrification ka oocytes.
5. IVF ephindiwe, uma okokuqala iqanda is hhayi ejwayele. Kulokhu, sinikeza vitrification hhayi ukuthatha impahla kathathu.

Vitrified oocytes donor - kuyini?

Kwezinye izimo, umusi oyinyumba ayikwazi okukhulelwa ingane, ngisho ngosizo kwesibeletho fertilization. Sikhuluma abesifazane nge umsebenzi kahle we gonads. Ngenxa yale njongo, amabhange abanikeli oocytes zakhiwa. Ngeshwa, azitholakali kuwo wonke amazwe. Zonke germ cells beya kukhona isimo vitrified, ukuze zingagcinwa. Izinkomba for yokusizwa kokusebenzisa oocytes yilezi:

1. zokugula kunqunywa Okushintshwe - wesibeletho ukwehluleka syndrome.
2. Ukungabikho oocytes ngenxa izici yobudala kwesimiso sokuzala (iminyaka engu-40 nangaphezulu).
3. Ukungakwazi zishukumisa ukukhiqizwa kwamaqanda ngenxa ukungenelela kuhlinzwa ezikhiqiza amaqanda, amakhemikhali okwelapha ukuchayeka.
4. Ingozi ingane nge isifo esidluliselwa ngofuzo.
5. Okuphindaphindayo IVF, ayizange yaba nemiphumela emihle ngenxa "eliphansi" amaqanda abo siqu.

Ubani ongaba oocytes donor vitrified: Izidingo

Wokuhlela Umndeni - lena isinqumo esinzulu, ikakhulukazi ezimweni lapho ezwa lo mbhangqwana uphoqelekile ukuba usebenzise donor amaseli. Ngaphambi uthola oocytes futhi okubeka ukuba vitrification, udokotela eyenziwa survey eziningi kokubili owesifazane impahla enikeziwe. Esinye sezimo elisungulwe osebeni ova eqandisiwe, kuyinto beqinisekisa ukungamethembi uRamaphosa. Okungukuthi, abazali abazi mayelana donor, futhi okuphambene nalokho. Okudingekayo ukuze abesifazane abakhetha oocytes yabo vitrified:

1. Ubudala: iminyaka 20-35.
2. Ukuswelakala kokusebenza ngokuhlanganyela izifo ukuphathwa kwabathintwayo kanye zofuzo.
3. Softly liphinyiselwe phenotype.
4. Ukuba khona lokuthi okungenani eyodwa ingane eyabo.
5. Imvume zobumfihlo.

Su vitrification

Ngaphambi kokuthi uqale ukumisa gametes zesifazane, aphethwe yi-ovulation ingqondo. Kuyadingeka ukuthi oocytes athile, kunokuba eyodwa. Ngemva yokuvuthwa evamile kwenzeka phakathi nge-ovulation kanye follicle isivuno esibelethweni eyodwa kuphela. Ukukhuthazwa wenziwa bathathe imithi yabo. Okulandelayo wenziwa a ultrasound okhalo. Uma uthola ukuthi ukukhiqizwa kwamaqanda isigcwaliseke futhi oocytes e esibelethweni eliningi eyanele, inqubo IVF iyaqhubeka. ukuvunwa iseli wenziwa khona ngaphansi ukubulawa kwezinzwa okuvamile. Ukuze lubhoboze inaliti siyishaye khona, lapho oocytes, yonke inqubo wenziwa ngaphansi ultrasound isiqondiso. Ngemva ngokuthatha impahla ukuze uqale esigabeni esilandelayo - kulondolozwe oocytes abonisa ukungavuthwa. Vitrification ka oocytes wenziwa emjikelezweni zemvelo ngenxa okusheshayo okokuqandisa indlela. Lena isici esiyinhloko le ndlela ukulondolozwa.

Izinzuzo vitrification ka oocytes

Njengoba sazi ukuthi vitrikatsii nqubo yaqala ukuhlanganisa kancane kancane esikhundleni ezinye izindlela kulondolozwe ova futhi imibungu, Kungcono hhayi kuphela odokotela kodwa futhi iziguli. Le ndlela has izinzuzo ezilandelayo:

1. The kungenzeka ukuhlehlisa sokukhulelwa. Ngenxa yokuthi i-oocytes futhi eqandisiwe egcinwe okwesikhathi eside (kuye ngokuthi isinqumo yowesifazane).
2. Ikhono kabusha inqubo IVF ngaphandle kokuthatha impahla izikhathi yesibili (noma ngaphezulu).
3. Ngekhono lokuhlala kombungu esibelethweni emva kokuhlinzwa ku nezindlala zangasese, welashwa ngemisebe nangamakhemikhali.

Wawuyini umehluko phakathi vitrification futhi cryopreservation amaqanda?

Njengoba yaziwa, izinqubo kriokenservatsii futhi vitrification ka oocytes kuyafana. Bobabili izindlela zenzelwe zeqhwa of oocytes kanye imibungu ukuze silondoloze nokusebenza sabo. Noma kunjalo, kukhona umehluko othize. Ngokuvamile, beya oocyte cryopreservation lilimele. Lokhu kungenxa yokuthi le ndlela kuhilela kancane okokuqandisa impahla. Ngenxa yalokho, i-cytoplasm ketshezi ibe iqhwa zinhlayiya. Lokho balimaza udonga cell. Indlela enhle kakhulu yokulungisa cryopreservation ka oocytes kuyinto vitrification. Amaphesenti eneqhwa lapho befeza yayo ephansi kakhulu, njengoba Ukupholisa ngomoya amaqanda kwenzeka ngokuphazima kweso. Lokho umehluko omkhulu kusuka cryopreservation ezibhekwa ubuchwepheshe obusha ukulondoloza oocytes eside esimweni iphila. Uyephuza owesifazane ebamba isikhathi eside kuphela indlela vitrification. Cryopreservation alinikezi Imiphumela enjalo kuze kube nini nanini.

Kangakanani-IVF ngemva vitrification ka oocytes?

Oocyte vitrification Kwenziwa yonke indawo. Zeqhwa of gametes futhi imibungu isikhathi eside wahlanganyela kuphela laboratories ezikhethekile kakhulu. Cishe njalo emtholampilo nale imishini has a yasebhange donor oocytes vitrified. Uma owesifazane uye wanquma ufisa ukuba akhulelwe ingane, amaseli incibilikiswa. Khona-ke sebenzisa amasu ICSI wenziwa kokukhulelwa. Umbungu okuholela is phansi culturing (3-5 izinsuku) futhi ezifakelwa ibe endometrium lelungiselelwe. Amaseli asele kungenziwa avundiswa futhi vitrified. Lapho ngomzamo ukugxilisa inqubo IVF iyaphindwa.

Vitrification ka oocytes: izibuyekezo odokotela

Okwamanje, vitrification futhi isitoreji oocytes kanye imibungu wenziwa kuyo yonke imizi ezinkulu imithi athuthukile. Uma le ndlela ihlolwe futhi sazibonakalisa, odokotela ukukhuluma ngayo kahle, ukwazi izinzuzo phezu kwezinye (ngaphambili) isitoreji izinqubo amaqanda. Afford ukumisa oocytes zabo kukhona abesifazane abaningi. Inqubo kubiza ruble angaba yizinkulungwane 12. Lokhu intengo ngoba vitrification. Izindleko wesitoreji kwamaseli eqandisiwe kuyinto ruble 1000 ngenyanga.