Kuyini igazi serum

Serum akulutho enjengayo plasma, elingenalo izakhi ifomu bese fibrin. Kumiswa It is ngenxa ukusabela ezithile zamakhemikhali. Thola serum ngezindlela ezimbili: zokuyithena fibrinogen calcium ion futhi ngenxa yemvelo igazi ekunqandeni.

Inqubo ekuwulungiseleleni ibizwa ngokuthi "defibrinirovanie". Nezobuchwepheshe, kunjengoba kulandela: imithambo yegazi eqoqwe angesabi ehlala, kuphenduke okuqinile fibrin ihlule. Kugcine ithumba egazi kanye isikhathi eside emi kancane kancane ekucindezela aphume yena ophuzi uketshezi. Lona serum igazi.

serum umbala ngenxa yokuba khona kuwo ethile bilirubin. ukwanda Isimo sayo sibonisa khona sombala umzimba iziyaluyalu. I evamile serum okusobala. Kodwa emva kokudla esinamafu kancane, okuyinto lula admixture amaconsi amafutha. Ukungezwani ebusweni the serum igazi kuyinto ephansi kakhulu kunalokho yamanzi.

Ubuningi ejwayelekile serum amaprotheni esiphakathi neyisishiyagalombili amaphesenti. Ngo ngezithako zawo iqukethe ikakhulukazi albumin (4.5-6.5%) kanye globulin (1.9-2.2%). Ukushintsha olinganise lezi zakhi amaprotheni yawo eguquguqukayo yabo ambalwa kukhona ezibaluleke kakhulu emtholampilo. Nokho, lolu daba akukaqondwa ngokugcwele.

Kwalungiswa izinkunga zokubona serum uhlala injalo ingashintshile ngaphansi kwethonya izici bokuphila, ezifana ukuchayeka hydrotherapy noma ukudla okunomsoco. Zila eside kungaholela ukwehla ezingeni amaprotheni ku serum. Kunalokho, umsebenzi kwemisipha cishe akunamthelela kwalungiswa izinkunga zokubona yayo.

Ukuwa kwe-inani iphrotheni serum igazi ebonwe izifo sibi ezithathelwanayo. Izinga wamaprotheni compounds wedwa kuza emuva evamile ngesikhathi yokutakula. Okungabalulwa sofuba, lapho isamba amaprotheni, ikakhulukazi globulin inyuka kakhulu.

Ngokuphathelene isicelo, evame ukusetshenziswa kakhulu igazi serum ngesikhathi kwamakhemikhali igazi ukuhlaziywa study wona izifo ezithathelwanayo, ukuhlola ukusebenza yokugoma futhi ukucacisa iqembu.

Okwamanje, izindlela ezimbili ezahlukene asetshenziswa kwezokwelapha, omunye igazi iqembu ejwayelekile sera. Ukuze ugweme amaphutha, sebenzisa serum kuphela esebenzayo nge titer okusezingeni eliphezulu. Ucwaningo lwenziwa egumbini lapho izinga lokushisa kufanele singadluli 25 degrees Celsius. Imiphumela kufanele ahlolwe ngaphambili kuka emva kwemizuzu 5 kusukela kuqale lolu cwaningo.

Izinsiza kusebenza lokhu Inqubo kanje. Ekuqaleni, kubalulekile ukucacisa titer ka dilution serum okuyinto akufanele kube ngaphansi kuka-oyedwa kwabathathu. Ngenxa yale njongo, ngamunye amashubhu esithathwayo ku amathonsi ezimbili ezinkulu, okuyinto zisetshenziswa a surface flat. Khona-ke, ngamunye wale amathonsi benezelwa ngamabomu inogruppnye erythrocyte u hlanganise serum. Ngemva kwemizuzu emihlanu, kunqunywa iconsi lokugcina, eyaqala agglutination. Lona dilution eliphakeme. Le nqubo ibizwa ngokuthi "serum titer gemagtlyutiniruyuschey".

Ngokulandelayo, ipuleti ingilazi noma esebenzisa pipettes lisetshenziswa iconsi ezinkulu serum ejwayelekile, bese induku ingilazi ixhunyiwe ngegazi layo amathonsi. Ngemva kwemizuzu emihlanu, ukuze ingxube ngayinye ingezwe iconsi ngu iconsi saline, bese uhlole imiphumela. Lena konke ohlobene ohlelweni lokunquma amaqembu ngegazi sera ejwayelekile.

Efingqa bonke ngenhla, kufanele kuphawulwe ukuthi namuhla serum - akuyona nje reagent kunesidingo, kodwa futhi isakhi esiyinhloko esebenzayo kwezidakamizwa eziningi asetshenziselwa ukwelapha inombolo enkulu yezifo ezithathelwanayo.